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Lose My Breath features the girls, in an alleyway, having a dance-off with the crowd as a showdown of an "in-fashion" and "street" Destiny's Child. Towards the end, the third Destiny's Child even "fiercer" takes the floor. Michelle represents the "in-fashion" Destiny's Child, Beyoncé represents the "fierce" Destiny's Child, and Kelly represents the "street" Destiny's Child.
The music video was nominated for the "Best Dance Video" category at the 2005 MTV Video Music Awards. The music clip is featured on the DualDisc editions of the albums #1's and Destiny Fulfilled and on the Bonus DVD of the Destiny Fulfilled Tour edition.